The liquid bioinoculant formulation technology goals to solve the drawbacks associated withsolid carrier based formulation like shorter shelf life, high contamination, poor quality, low field performance and processing the carriers. Thisstudy was conducted to formulate and determine the effect of liquid biofertilizers containing different cell protectants on the strain Azotobacter chroococcum. The cell protectants used were glycerol (1%), polyethylene glycol (PEG, 2%),Polyvinyl alcohol (PVA,

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... %) and Gum Arabic (GA, 0.3%). The 2 treatments without addition of cell protectants i.e. containing only broth were maintained as check.The formulated liquid biofertilizers of Azotobacter were stored in BOD incubator at 28±2 °C for a period of 15 days to see the effect of cell protectants on the mass multiplication of cells. The colony forming units (CFU) were calculated on 0 th dayand on 15 th day. Liquid Azotobacter bioinoculants formulated with glycerol (1%) + PEG (2%) promoted long term survival followed by gum arabic (0.3%), PEG (2%) and glycerol (1%) + PVA (0.5%). The results of the present study clearly indicated that the liquid formulation of Azotobacter could be used more effectively to maintain the cells viability for long time.