Fluorescein Chemiluminescent Assay of Glucose in Serum Using Glucose Oxidase and Horseradish Peroxidase

Tadashi SEGAWA, Asao KAKIZAKI, Tamio KAMIDATE, Hiroto WATANABE
1992 Analytical Sciences  
Glucose in serum was successfully determined by the simultaneous coupling of a glucose-glucose oxidase (GOD) reaction with a fluorescein (FL)-chemiluminescent (CL) reaction catalyzed by horseradish peroxidase. The FL-CL reaction could be conducted at pH 7.0, thus allowing the in situ detection of hydrogen peroxide generated during the glucose-GOD reaction. The CL emission lasted for about 3 min, and the calibration curve was linear over a glucose concentration range of 5.OX 10-8 to 1.0X105 M.
more » ... e relative standard deviation at 5.OX 10-' M glucose was 1.8% (n=5). The present method was not subject to any interference from components normally present in serum. The results of glucose assays in two control sera were in good agreement with those conducted by the colorimetric method.
doi:10.2116/analsci.8.785 fatcat:lskxo64n4rhdrl53rgf5uz4esm