Supplemental Table 2-Target prediction via TargetscanHuman 7.1.pdf from Reduced miR-125a-5p level in non-small-cell lung cancer is associated with tumour progression

Yi Ren, Hongxu Liu, Yegang Ma, Changhao Liu, Pengfei Li, Tao Yu
2018 Figshare  
Emerging evidence suggests that microRNAs (miRNAs) serve an important role in tumourigenesis and development. Although the low expression of miR-125a-5p in non-small-cell lung cancer (NSCLC) has been reported, the underlying mechanism remains unknown. In the current study, the low expression of miR-125a-5p in NSCLC was verified in paired cancer tissues and adjacent non-tumour tissues. Furthermore, the CpG island in the miR-125a-5p region were hypermethylated in the tumour tissues. And the
more » ... ethylation was negatively correlated with the miR-125a-5p expression. Target gene screening showed that the histone methyltransferase Suv39H1 was one of the potential target genes. In vitro studies showed that miR-125a-5p could directly suppress the Suv39H1 expression and decrease the H3K9me3 levels. On the other hand, the Suv39H1 could induce demethylation of miR-125a-5p, resulting in re-activation of miR-125a-5p. What is more, overexpessing miR-125a-5p could also self-activate the silenced miR-125a-5p in NSCLC cells, which suppressed cell migration, invasion and proliferation in vitro and inhibited cancer progression in vivo. Thus, we uncovered here that the epigenetic silenced miR-125a-5p could be self-activated through targeting Suv39H1 in NSCLC, suggesting that miR-125a-5p might not only have the potential prognostic value as a tumour biomarker but also be potential therapeutic targets in NSCLC.
doi:10.6084/m9.figshare.7110182 fatcat:joyuuw6i7jfnloywdw4mbguiye