Commonality and Biosynthesis of theO-Methyl Phosphoramidate Capsule Modification inCampylobacter jejuni

David J. McNally, Marc P. Lamoureux, Andrey V. Karlyshev, Laura M. Fiori, Jianjun Li, Gillian Thacker, Russell A. Coleman, Nam H. Khieu, Brendan W. Wren, Jean-Robert Brisson, Harold C. Jarrell, Christine M. Szymanski
2007 Journal of Biological Chemistry  
In this study we investigated the commonality and biosynthesis of the O-methyl phosphoramidate (MeOPN) group found on the capsular polysaccharide (CPS) of Campylobacter jejuni. High resolution magic angle spinning NMR spectroscopy was used as a rapid, high throughput means to examine multiple isolates, analyze the cecal contents of colonized chickens, and screen a library of CPS mutants for the presence of MeOPN. Sixty eight percent of C. jejuni strains were found to express the MeOPN with a
more » ... the MeOPN with a high prevalence among isolates from enteritis, Guillain Barré, and Miller-Fisher syndrome patients. In contrast, MeOPN was not observed for any of the Campylobacter coli strains examined. The MeOPN was detected on C. jejuni retrieved from cecal contents of colonized chickens demonstrating that the modification is expressed by bacteria inhabiting the avian gastrointestinal tract. In C. jejuni 11168H, the cj1415-cj1418 cluster was shown to be involved in the biosynthesis of MeOPN. Genetic complementation studies and NMR/ mass spectrometric analyses of CPS from this strain also revealed that cj1421 and cj1422 encode MeOPN transferases. Cj1421 adds the MeOPN to C-3 of the ␤-D-GalfNAc residue, whereas Cj1422 transfers the MeOPN to C-4 of D-glycero-␣-Lgluco-heptopyranose. CPS produced by the 11168H strain was found to be extensively modified with variable MeOPN, methyl, ethanolamine, and N-glycerol groups. These findings establish the importance of the MeOPN as a diagnostic marker and therapeutic target for C. jejuni and set the groundwork for future studies aimed at the detailed elucidation of the MeOPN biosynthetic pathway. 5 The abbreviations used are: CPS, capsular polysaccharide; MeOPN, O-methyl phosphoramidate CH 3 OP(O)(NH 2 )(OR); CE-ESI/MS, capillary electrophoresis electrospray ionization mass spectrometry; HMQC, heteronuclear multiple-quantum coherence; HMQCTOXY, heteronuclear multiple-quantum coherence total correlation spectroscopy; HR-MAS NMR, high resolution magic angle spinning nuclear magnetic resonance spectroscopy; HSQC, heteronuclear single-quantum coherence; NOESY, nuclear Overhauser effect spectroscopy; Kan, kanamycin.
doi:10.1074/jbc.m704413200 pmid:17675288 fatcat:soevvsxh4ngijgd7ta6td7dvtm