In vitro susceptibility of rainbow trout fin cells to viral haemorrhagic septicaemia virus

A Estepa, D Frias, JM Coll
1993 Diseases of Aquatic Organisms  
Cells isolated from rainbow trout fins Oncorhynchus mykiss, Walbaum were propagated and subcultured a s fibroblast-like. Viral haemorrhagic septicaemia virus (VHSV) replicated in the cultured fin cells as demonstrated by fin cell lysis, posihve immunofluorescence with polyclonal anti-VHSV, and Increase in titre as measured by either infection of f~s h cell lines or by sandwich ELISA with anti-VHSV monoclonal antibodies. The possible use of these fln cell cultures as VHSV-antigen presenting cells for immunological studies is briefly discussed.
doi:10.3354/dao015035 fatcat:byd2wvutvfekvget334yqahy3u