A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae [article]

Jidapas (My) An-adirekkun, Cameron J. Stewart, Stephanie H. Geller, Michael T. Patel, Justin Melendez, Benjamin L. Oakes, Marcus B. Noyes, Megan N. McClean
2019 bioRxiv   pre-print
AbstractOptogenetic tools for controlling gene expression are ideal for tuning synthetic biological networks due to the exquisite spatiotemporal control available with light. Here we develop an optogenetic system for gene expression control and integrate it with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae. We reconstitute activity of a split synthetic zinc-finger transcription factor (TF)
more » ... iption factor (TF) using light-induced dimerization. We optimize function of this split TF and demonstrate the utility of the toolkit workflow by assembling cassettes expressing the TF activation domain and DNA-binding domain at different levels. Utilizing this TF and a synthetic promoter we demonstrate that light-intensity and duty-cycle can be used to modulate gene expression over the range currently available from natural yeast promoters. This work allows for rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.
doi:10.1101/663393 fatcat:7mch4g3ncfebbo2t5qxo5mlo3e