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Muscles [diaphragm, extensor digitorum longus (EDL), tibialis anterior, and soleus] were dissected from at least three sAnk1 KO and three control B6 mice of 4 -6 mo (young mice) and of 12-15 mo of age (old mice). Samples were then frozen in liquid nitrogen and cryoprotected with Tissue-Tek II OCT compound (Sakura Finetek Europe, Leiden, The Netherlands). Transverse or longitudinal sections 8 m thick were cut with a Leica cryostat (CM 1850, Leica Microsystem, Wetzlar, Germany) and fixed with 3%doi:10.1152/ajpcell.00090.2014 pmid:25354526 fatcat:rkgyd2ieq5gk5nfawpv2hnrdsy