Cloning and functional analysis of Phyllostachys edulis MYB transcription factor PeMYB2

Dong-Chang XIAO, Zhi-Jun ZHANG, Ying-Wu XU, Li YANG, Feng-Xue ZHANG, Chao-Li WANG
2013 Hereditas (Beijing)  
MYB-type transcription factor is one of the largest families in plants, which plays important roles in accepting stress signals from environment and regulating the expression of stress-tolerant genes. In this paper, using homologous cloning and RACE technology, a MYB-type transcription factor, designated PeMYB2, was cloned from Phyllostachys edulis. The results of bioinformatics showed that PeMYB2 is a typical R2R3-MYB. It contained two tandem repeats in its N-terminus, and a membrane protein
more » ... F3651 in its C-terminus. In addition, phylogenetic analysis indicated that PeMYB2 shared the highest homology with 85.98% to OsMYB18 protein from Oryza sativa spp. Japonica. In addition, a yeast one-hybrid assay showed that PeMYB2 could activate the expression of downstream genes. After PeMYB2 was
doi:10.3724/sp.j.1005.2013.01217 pmid:24459895 fatcat:iub7winkkjeillswwzzoct4wcy