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Two new α-pyrones, fupyrones A and B (1 and 2) and a biosynthetically related known α-pyrone, 4-methyl-5,6-dihydro-2H-pyran-2-one (3), were isolated from an endophytic fungus, Fusarium sp. F20, harbored in the stems of the Chinese medicinal plant Mahonia fortunei. Their structures were determined on the basis of spectroscopic data including MS and NMR. The antibacterial efficacies of compounds 1–3 were evaluated against four selected Gram-positive and Gram-negative bacteria, and their quorumdoi:10.6084/m9.figshare.7542842.v1 fatcat:za57ys2ox5bjlfcgmfn3wguacy