Evaluation of Biocompatibility of Chitosan Films from the Mycelium of Aspergillus niger in Connective Tissue of Rattus norvegicus

Silva DJB Zuluaga F
2015 Journal of Molecular and Genetic Medicine  
Chitosan was extracted from Aspergillus niger mycelium and characterized by several techniques (FTIR, 1H-NMR y 13C-CP-MAS-NMR, TGA, DSC and XRD) with a reaction yield of 5.02%, and its average viscosity molecular weight was 1.52 x 105 g/mol. Deacetylation degree was determined by elemental analysis and 1H-NMR, being around 75%. Chitosan films were obtained by film casting, using glycerol and oleic acid as plasticizers, the chitosan films were characterized by SEM and XRD. Degradability in vitro
more » ... tests under physiological conditions, mechanical tests, and biocompatibility tests on Rattus norvegicus were run, followed by histological studies of connective tissue samples from the site where the films were implanted finding complete absorption of the material and no damage to nearby tissues.
doi:10.4172/1747-0862.1000174 fatcat:yuv37sb3orcn5j6bxrcuin2xju