Posttranslational Modifications in an Insect Cell-Free Protein Synthesis System and Their Identification by MALDI-TOF MS

T. Ezure, T. Suzuki, M. Shikata, E. Ando, T. Utsumi, S. Tsunasawa
2008 Journal of Proteomics & Bioinformatics  
and geranylgeranylated protein respectively, were synthesized using the insect cell-free protein synthesis system with or without addition of a specific substrate for each protein modification, such as myristoyl-CoA, farnesyl pyrophosphate, and geranylgeranyl pyrophosphate. A nalyses of these proteins by MALDI-TOF MS indicate that the insect cell-free protein synthesis system, as is the case with the rabbit reticulocyte lysate system, possesses N-myristoyltransferases and prenyltransferases.
doi:10.4172/jpb.s1000177 fatcat:siwgh3o7yvdg3emx5y74ztlhee