SEM Sample Prep v1 (protocols.io.w9ufh6w) [dataset]

Tim D, Beth Orcutt
2019 protocols.io  
The SEM can be used to directly image biofilms on solid surfaces such as polished rock chips or collected crustal samples. Seawater or fluid bacteria can be imaged after filtering the sample onto a 0.2 μM filter. Re a ge nts: Re a ge nts: 4% Paraformaldehyde in 1x PBS (VWR 102091-888) Hexamethyldisilane (HMDS) (VWR TCH0638) Ethanol, 100% molecular biology grade MilliQ water, sterile 1x PBS, autoclaved, filtered. Suppl i e s: Suppl i e s: · Desiccator with fresh dessicant · 1.5ml eppitubes,
more » ... 5ml eppitubes, sterile · Forceps · Lighter or Bunsen burner · Plastic syringes, sterile · 0.2um-mesh syringe filters, sterile · Small petri dishes, sterile · Scissors · Sharpie marker · 12.5 mm aluminum pin stubs -Electron Microscopy Sciences #75210 ($17/pack 50) · Aluminum-nickel conductive tape -Electron Microscopy Sciences #77813 ($28) · Double-sided carbon adhesive disc, 12mm -Electron Microscopy Sciences #77825-12 ($17/pack 100) · Stub holder box -Electron Microscopy Sciences #76500 ($4/box) Hexamethyldisilane (HMDS) is volatile and flammable, do not open the bottle outside of the hood. It is stored in the flammable's cabinet in A010. Wear gloves while handling. Paraformaldehyde (PFA) is toxic and should only be opened inside a BSC or Fume Hood. Wear gloves while handling.
doi:10.17504/protocols.io.w9ufh6w fatcat:jsz4i3enzje3xefwciyp5gr2te