Transcription Activator-like Effectors: A Toolkit for Synthetic Biology

Richard Moore, Anita Chandrahas, Leonidas Bleris
2014 ACS Synthetic Biology  
Transcription activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria to aid the infection of plant species. TALEs assist infections by binding to specific DNA sequences and activating the expression of host genes. Recent results show that TALE proteins consist of a central repeat domain, which determines the DNA targeting specificity and can be rapidly synthesized de novo. Considering the highly modular nature of TALEs, their versatility, and the ease of constructing
more » ... e proteins, this technology can have important implications for synthetic biology applications. Here, we review developments in the area with a particular focus on modifications for custom and controllable gene regulation.
doi:10.1021/sb400137b pmid:24933470 pmcid:PMC4210167 fatcat:3k6w4t5a5jd3fppqn42bahao2i