Plant Regeneration Through Somatic Embryogenesis in Two Cultivars of Pineapple (Ananas Comosus L.)
Pineapple production is mostly constrained by unavailability of high-performance suckers. However, somatic embryogenesis (SE) have been revealed the most rapid and controllable method for Pineapple propagation than conventional sucker production methods. The aim of this study was to evaluate the responses of two cultivars of pineapple regenerated through somatic embryogenesis. Calli were induced from crown leaf and plantlets leaf of Smooth Cayenne and Sugar Loaf cultivars. Murashige and Skoog
... rashige and Skoog base medium supplemented with vitamins B5 and different plant growth regulators combinations. BAP and / or 2,4-D have been added to base medium for calli maturation. BAP and GA3 have been added for plant elongation. The results indicated a significant influence of type of explant and copper on callus induction in pineapple cultivars. Likewise, the medium MS with NAA (0.5 mg/l) + BAP (1mg/l) has a highly significant influence with 8.8 mature somatic embryos per explant. Also, the growth regulator combinations and the cultivars have significantly influenced somatic embryos regeneration with a high rate of 55.25% plantlets using the hormonal combination BAP (3 mg/l) + GA3 (2 mg/l) for the smooth Cayenne. Leaves from plantlets constitute the explants to be used for callus induction in pineapple. The combination of BAP (1 mg/l) + copper (2 mg/l) + Picloram (6 mg/l or 12 mg/l) on Murashige and Skoog medium supplemented with vitamins B5 was favorable somatic embryos regeneration of pineapple. The protocol developed is a key study for successful mass propagation and genetics transformation of pineapple.