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Genome-Wide CRISPR-Cas9 Screen Identifies MicroRNAs That Regulate Myeloid Leukemia Cell Growth
2016
PLoS ONE
Mammalian microRNA expression is dysregulated in human cancer. However, the functional relevance of many microRNAs in the context of tumor biology remains unclear. Using CRISPR-Cas9 technology, we performed a global loss-of-function screen to simultaneously test the functions of individual microRNAs and protein-coding genes during the growth of a myeloid leukemia cell line. This approach identified evolutionarily conserved human micro-RNAs that suppress or promote cell growth, revealing that
doi:10.1371/journal.pone.0153689
pmid:27081855
pmcid:PMC4833428
fatcat:bkvfpr73jbdkvn3ifgbkhccjj4