Chromosomal Aberrations, Sister Chromatid Exchanges and Nuclear Status of Immature Oocytes in Relation to Age of Dromedary Camels

Karima Gh. M. Mahmoud, T. H. Scholkamy, A. Farghaly, M. F. Nawito
The present study was conducted to analyse the chromosomes of blood culture and oocyte chromatin quality at the time of recovery in dromedary camels in relation to age. Twelve young (about one year) and twelve adult (4-10 years old) female camels were used. These animals with unknown reproductive history were slaughtered in Kerdasa abattoir (Giza province, Egypt). Blood samples were collected via sterile syringes from camels before slaughtering for chromosomal analysis. Oocytes from ovaries of
more » ... oth ages were aspirated from small antral follicles 1 to 5 mm in diameter and classified according to their quality into four categories. Nuclear status of cumulus oocytes complexes (COCs) were evaluated directly after collection. The results indicated that, the frequencies of chromosomal abnormalities and sister chromatid exchanges (SCE'S) were increased significantly (pϽ0.05) with age. An increase in structural aberrations could be observed. There were no significant differences between young and adult camels in total number and quality of oocytes. Statistically significant (pϽ0.01) differences were between percentages of germinal vesicle breakdown (GVBD) and Germinal vesicle (GV) in young and adult camels. It is concluded that, the increase of age may have significant effects on structural chromosomal aberrations, SCE'S and meiotic stages of immature camel oocytes but not on the number and quality of oocytes.
doi:10.1508/cytologia.70.295 fatcat:mimp44luefg3dnh5tnvqlmaydq