Enhanced excitability and suppression of A-type K ϩ current of pancreas-specific afferent neurons in a rat model of chronic pancreatitis. Chronic pancreatitis (CP) is a relatively common disorder, characterized by glandular insufficiency and chronic, often intractable, pain. The mechanism of pain in CP is poorly understood. We have previously developed a model of trinitrobenzene sulphonic acid (TNBS)-induced CP that results in nociceptive sensitization in rats. This study was designed to

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... changes in the excitability and alteration of voltage-gated K ϩ currents of dorsal root ganglia (DRG) neurons innervating the pancreas. CP was induced in adult rats by an intraductal injection of TNBS. DRG neurons innervating the pancreas were identified by 1,1Ј-dioleyl-3,3,3Ј,3-tetramethylindocarbocyanine methanesulfonate fluorescence labeling. Perforated patch-clamp recordings were made from acutely dissociated DRG neurons from control and TNBS-treated rats. Pancreas-specific DRG neurons displayed more depolarized resting potentials in TNBS-treated rats than those in controls (P Ͻ 0.02). Some neurons from the TNBS-treated group exhibited spontaneous firings. TNBS-induced CP also resulted in a dramatic reduction in rheobase (P Ͻ 0.05) and a significant increase in the number of action potentials evoked at twice rheobase (P Ͻ 0.05). Under voltage-clamp conditions, neurons from both groups exhibited transient A-type (I A) and sustained outward rectifier K ϩ currents (IK). Compared with controls, the average I A but not the average IK density was significantly reduced in the TNBS-treated group (P Ͻ 0.05). The steadystate inactivation curve for I A was displaced by ϳ20 mV to more hyperpolarized levels after the TNBS treatment. These data suggest that TNBS treatment increases the excitability of pancreas-specific DRG neurons by suppressing I A density, thus identifying for the first time a specific molecular mechanism underlying chronic visceral pain and sensitization in CP. dorsal root ganglion; inflammation; visceral pain; trinitrobenzene sulfonic acid; membrane properties Address for reprint requests and other correspondence: P. J. Pasricha,