Corticoids and body and organ weights nitrogen balance, and enzymes
Journal of Biological Chemistry
481 by guest on March 24, 2020 http://www.jbc.org/ Downloaded from 482 EFFECTS OF CORTICOIDS pared diet composed of casein 16.7, sucrose 61.2, hydrogenated vegetable oil 7.4, dry brewers' yeast (Fleischmann's No. 2019) 9.2, Cellu flour 1.8, Wesson's salt mixture 3.7 (6) , and three times per week a supplement of 1 drop of cod liver oil and 1 drop of a 34 per cent tocophero12 concentrate of wheat germ oil diluted lo-fold with Wesson oil. The prepared diet was fed in a special feeder (7) so that
... feeder (7) so that the amount eaten could be determined, and was used in all of the nitrogen balance studies. The animals were fed and weighed just before the urine collections. Hormone Treatment-The experiments were begun 40 to 60 days after castration. They were either acute in accordance with the Reinecke-Ken-da11 test (8) and the modification of Olson et al. (9) or chronic by daily injections for 25 days and the subcutaneous implantation of pellets of the steroids for various periods of time. The pellets were cylindrical in shape and weighed approximately 14 mg. each (10). The control animals were sham operated. The mice for the different groups in each series of experiments were paired according to body weight. Autopsy and Analyses-In the acute experiments the mice were killed by spreading the vertebrae at the neck to sever the spinal cord. The liver was immediately removed and approximately one-half used for glycogen determination by the Good-Kramer-Somogyi technique (ll), except that the hydrolyzed glycogen was neutralized to phenolphthalein (12). The improved Somogyi method (13) was used to determine the reducing substances as glucose. The remainder of the liver was homogenized for the enzyme determinations as previously described (5, 14) . In the chronic experiments food but not water was removed from the mice 19 to 24 hours before autopsy. The spinal cord of the mice was severed as above and the animals were immediately bled to death by cutting the blood vessels of the neck. Approximately one-half of the liver and one kidney were homogenized for the enzyme determinations and the rest of the liver, the other kidney, and the remaining tissues were saved for analyses. The organs were weighed on a Roller-Smith torsion balance. Nitrogen was determined by the micro-Kjeldahl procedure.