Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products

Édilla Ribeiro dos Santos, Zozilene Nascimento Santos Teles, Núria Mariana Campos, Diogo Angeli Jacinto de Souza, Aline Simões da Rocha Bispo, Rodrigo Pires do Nascimento
2012 Brazilian Archives of Biology and Technology  
Approximately 1.5 trillion tons are the estimated yearly biomass production, making it an essentially unlimited source of raw material for environmentally friendly and biocompatible products transformed by microorganism, specially fungi and actinomycetes. Several lignocellulosic residues, such as sisal waste and sugarcane bagasse contain starch in their structures which could become important sources for the production of amylases. This study evaluated the production of amylolytic enzymes using
more » ... Streptomyces sp. SLBA-08 strain, isolated from a semi-arid soil, according to their ability to grow on soluble starch as the sole carbon source. The effect of the carbon source (sisal waste and sugarcane bagasse) on α-amylase production was studied using submerged cultivations at 30 °C. The highest level of α-amylase activity corresponded to 10.1 U. mL -1 and was obtained using sisal waste (2.7%) and urea (0.8%) in submerged fermentation after 3 days of cultivation. The partial characterization showed the best αamylase activity at 50ºC and pH 7.0. These results are of great importance for the use of sisal waste as a substrate for biotechnological proposes.
doi:10.1590/s1516-89132012000500020 fatcat:4kdqmhggozcplfvvuxmkhkom2e