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DEVELOPMENT AND VALIDATION OF A STABILITY INDICATING RP-UPLC METHOD FOR THE SIMULTANEOUS QUANTIFICATION OF SOFOSBUVIR AND VELPATASVIR IN FINISHED DOSAGE FORM
A simple, rapid, accurate, precise and economical reverse phase Ultra performance liquid chromatographic method was developed for simultaneous quantification of two anti-viral drugs, viz., Sofosbuvir and Velpatasvir. The separation of both the drugs was achieved on Endeavorsil C 18 column (2.1 × 50 mm, 2.5 μm particle size) as a mobile phase with phosphate buffer (at pH 3): Acetonitrile (50:50 v/v). The flow rate was 0.3 ml/min and detection was done at 240 nm based on isobestic point. Thedoi:10.5281/zenodo.2531170 fatcat:xujehgjhr5fhpaifq42drevovq