Molecular typing of Salmonella from Sergipe, Northeastern Brazil, showing the epidemiological relationship between poultry and human infection

P.B.P. Góis, M.R.P. Carneiro, S. Jain, M.I.S. Santos, M.V.A. Batista, A.L. Cândido
2015 Genetics and Molecular Research  
Randomly amplified polymorphic DNA (RAPD) has been widely used for epidemiological and phylogenetic purposes owing to its rapidity and efficiency. The aim of this study was to perform genome typing of Salmonella samples isolated from different sources by RAPD profiling. Thirty-three Salmonella samples from the bacterial collection and two standard samples were used. RAPD profiling was conducted using six primers of the Ready-To-Go RAPD system. The amplified products were electrophoresed on 5%
more » ... lyacrylamide gel and silver-stained. RAPD analysis resulted in reproducible and stable banding patterns and showed high genetic diversity among the isolated strains. The Primer P1-generated dendrogram showed an epidemiologic relationship between the human ©FUNPEC-RP Genetics and Molecular Research 14 (3): 11389-11395 (2015) P.B.P. Góis et al. and poultry isolated samples, highlighting the usefulness of RAPD for molecular typing and epidemiological studies.
doi:10.4238/2015.september.25.6 pmid:26436380 fatcat:fuste522vfh35irsaxlr5s6tuy