Genetic diversity analysis of Sahiwal and Tharparker breeds of cattle (Bos indicus) by microsatellite markers

Sanjeev Singh, Sarita Kankoriya, Sudhir Kumar Jain, Hemlata Jain, Satyendra Pal Singh
2014 Indian Journal of Animal Research  
15 microsatellite loci were investigated for studying the genetic polymorphism in Sahiwal (30) and Tharparker (30) breeds of cattle. Polymerase chain reactions (PCR) were carried out and amplified products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. Out of the 15 microsatellite loci, 14 were found polymorphic. The number of alleles ranged from 3 to 7. The overall polymorphic information content (PIC) values ranged from 0.293 to 0.775. Wright's F statistics was
more » ... F statistics was carried out to determine the between breed (Fst) and within breed (Fis) genetic divergence. Fst value of 0.128 (P< 0.01) revealed moderate genetic diversity between these two breeds. The Fis values were -0.08 and -0.01 in Sahiwal and Tharparker breeds, respectively. The results indicated that the microsatellite markers can be used for genetic diversity analysis in other cattle breeds and identification of individual animals.
doi:10.5958/0976-0555.2014.00004.1 fatcat:rhyqxjjkabhgxnmvpzyso7s7pe