The potential of a single injection of hUCMSCs in relevant testis injury and related severity of DSS induced acute and chronic colitis [post]

Yu-Lung Chang, Huei-Yu Lo, Shun-Ping Cheng, Kuo-Ting Chang, Xiu-Fang Lin, Kuo-sheng Liao, Jing-Long Huang, Ming-Fa Hsieh, Chin-Kan Chan
2021 unpublished
Background A single injection of human umbilical mesenchymal stem cells (hUCMSCs) can improve dextran sulfate sodium (DSS)–induced acute colitis and decrease progression of acute to chronic colitis. We report the therapeutic efficacy of hUCMSCs in acute and chronic colitis-related testicular injury. Methods Male C57BL/6JNarl mice were divided into control, phosphate-buffered saline 1 (PBS1), PBS2, hUCMSCs 1 (SC1), and SC2 treated groups. Colitis were induced in the mice using 3% DSS. The mice
more » ... 3% DSS. The mice in the SC1 and SC2 groups underwent a single injection of hUCMSCs. Results The ratio of spermatogenesis impairment of mice in the stem cell groups was lower than that in the PBS groups on days 8 and 25 (P=.029, .043). The overall testicular function impairment and severity of colitis in the PBS2 group were lower than those in the PBS1 group on days 8 and 25 (P=.031, .016). The overall testicular function impairment and severity of colitis in the SC2 group were lower than those in the SC1 group on days 8 and 25 (P=.031, .016). Conclusions Disease activity of acute colitis is a good predictor of severity and prognosis of testicular injury in mice with colitis. hUCMSCs can reduce the ratio of testicular function impairment in acute and chronic colitis.
doi:10.21203/rs.3.rs-270686/v1 fatcat:6v52y2ybsbdbfcfckh2eg7xn44