Matrix metalloproteinase-1 suppression and type-1 procollagen synthesis promoting effects of Uncaria gambir

Jaemin Lee, Dong-Hee Kim, Eun-Woo Kim, Hyun Ju Kwon, Byung Woo Kim, Jae-Hyon Cho, Hyun-Joo Kim, Tae Hoon Kim
2017 Korean Journal of Food Preservation  
Collagen synthesis is decreased and matrix metalloproteinase-1 (MMP-1) levels are increased in naturally aged human skin, and these alterations cause changes such as skin wrinkling and decreased elasticity. As a part of our ongoing search for bioactive ingredients, MMP-1 inhibitory and type-1 procollagen synthesis inducing activities of aqueous methanolic extract of manufactured gambir product from Uncaria gambir were investigated in in vitro bioassay systems. In addition, total phenolic
more » ... s were quantified using a spectrophotometric method. Among tested samples, 40% MeOH eluate from 80% methanolic extract of manufactured U. gambir using open column chromatography packed with Diaion HP-20 resin showed significant MMP-1 inhibitory activities with an IC50 value of 15.6±1.3 μg/mL. Furthermore, type-1 procollagen synthesis promoting property of 40% MeOH eluate (IC 50 value; 6.9±0.7 μg/mL) from 80% methanolic extract of manufactured gambir was higher than other eluates. Additionally, the present investigation revealed that 40% MeOH eluate of manufactured gambir product contained a high level of total phenolic compounds. The result suggests a distinct relationship between anti-wrinkle activity and total phenolic contents, and manufactured gambir product could be considered a new effective source of natural bioactive ingredients. Systematic investigation of manufactured gambir product will be performed for further development of its biological properties. Key words:Uncaria gambir, anti-wrinkle activity, matrix metalloproteinase-1, type-1 procollagen, phenolics 서 론 1)
doi:10.11002/kjfp.2017.24.1.93 fatcat:2xachbybwfhvhdyskft7qwtcpe