αv-Integrin Utilization in Human β-Cell Adhesion, Spreading, and Motility

Thomas Kaido, Brandon Perez, Mayra Yebra, Jesse Hill, Vincenzo Cirulli, Alberto Hayek, Anthony M. Montgomery
2004 Journal of Biological Chemistry  
The role of individual integrins in human ␤-cell development and function is largely unknown. This study describes the contribution of ␣ v -integrins to human ␤-cell adhesion, spreading, and motility. Developmental differences in ␣ v -integrin utilization are addressed by comparing the responses of adult and fetal ␤-cells, and vitronectin is used as a substrate based on its unique pattern of expression in the developing pancreas. Fetal and adult ␤-cells attached equally to vitronectin and
more » ... in ␣ v ␤ 5 was found to support the adhesion of both mature and immature ␤-cell populations. Fetal ␤-cells were also observed to spread and migrate on vitronectin, and integrin ␣ v ␤ 1 was found to be essential for these responses. In contrast to their fetal counterparts, adult ␤-cells failed to either spread or migrate and this deficit was associated with a marked down-regulation of ␣ v ␤ 1 expression in adult islet preparations. The integrin ␣ v ␤ 3 was not found to support significant ␤-cell attachment or migration. Based on our findings, we conclude that integrins ␣ v ␤ 5 and ␣ v ␤ 1 are important mediators of human ␤-cell adhesion and motility, respectively. By supporting fetal ␤-cell migration, ␣ v ␤ 1 could play an important role in early motile processes required for islet neogenesis.
doi:10.1074/jbc.m308425200 pmid:14766759 fatcat:tb22yhku4bgonfkdzvgka2lmbe