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Ratiometric intracellular calcium imaging in the isolated beating rat heart using indo-1 fluorescence

Otto Eerbeek, Egbert G. Mik, Coert J. Zuurbier, Martijn van 't Loo, Cees Donkersloot, Can Ince
2004 Journal of applied physiology  

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Eerbeek, Otto, Egbert G. Mik, Coert J. Zuurbier, Martijn van 't Loo, Cees Donkersloot, and Can Ince. Ratiometric intracellular calcium imaging in the isolated beating rat heart using indo-1 fluorescence. ties in intracellular calcium (Ca i 2ϩ ) handling have been implicated as the underlying mechanism in a large number of pathologies in the heart. Study into the relation between Ca i 2ϩ behavior and performance of the whole heart function could provide detailed information into the cellular
more » ... s of heart function. In this study we describe an optical ratio imaging setup and an analysis method for the beat-to-beat Ca i 2ϩ Address for reprint requests and other correspondence: O. Eerbeek,
doi:10.1152/japplphysiol.01125.2003 pmid:15208283 fatcat:d5wg5shv35billzzk2jeofpxiq
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Otto Eerbeek, Egbert G. Mik, Coert J. Zuurbier, Martijn van 't Loo, Cees Donkersloot, Can Ince. "Ratiometric intracellular calcium imaging in the isolated beating rat heart using indo-1 fluorescence." Journal of applied physiology 97.6 (2004) 2042-2050