Development and characterisation of microsatellite markers for the fungus Lasiodiplodia theobromae

José Emilson Cardoso, Michael James Wilkinson
2008 Summa Phytopathologica  
Lasiodiplodia theobromae is an important fungal pathogen of higher plants from tropical and sub-tropical regions. The fungus infects divergent hosts in a wide range of environmental conditions, suggesting that it is highly variable. The aim of this study was to develop new polymorphic microsatellite markers from a Brazilian isolate of L. theobromae that can be used in population studies of this and related fungi. The nine microsatellite markers developed included six that revealed allelic
more » ... rphisms among nine isolates of the disease collected from infected plants in Brazil. Preliminary evaluation of the markers suggested substantial genetic variability among Brazilian L. theobromae populations. These markers have potential utility for evolutionary and epidemiologic studies of this fungus.
doi:10.1590/s0100-54052008000100010 fatcat:ui6mfwzoh5eezdshv2dzw2kbmi