X-ray Diffraction Pattern of Nerve Myelin: A Method for Determining the Phases

M. F. Moody
1963 Science  
Ten days later the fetuses were delivered, bled, and autopsied. The rationale behind this experiment was as follows: If the fetus could be shown to reject a skin homograft in the normal way in the presence of persisting rabbit anti-sheep globulins, then the conclusion would be almost inevitable that circulating antibody does not play an obligatory role in this process. Under these conditions, fetal antibody globulins, even if produced, should be unable to reach the graft site. If, on the other
more » ... . If, on the other hand, graft rejection were inhibited or altered by the rabbit antisheep globulins, then circulating antigraft antibody must be implicated in the rejection process according to a similar line of reasoning. In every instance, the grafts were rejected in an apparently normal fashion. In addition, each fetus showed a persisting level of circulating rabbit y-globulin at the end of the 10-day period. Of the animals that had received rabbit anti-sheep globulins, only one showed persistence of detectable antisheep 7S y-globulin and anti-sheep /32Mglobulin activity in its serum by tanned cell hemagglutination assay. Although the titers were only moderate (1:8 in each case), satisfactory negative controls and standards render the data significant. It became apparent that at this point (10 days after injection) the rabbit anti-sheep globulins were being cleared rapidly from the fetal circulation, and that we were fortunate in finding persisting anti-globulin activity even in the one fetus. In addition to the normal catabolism of rabbit protein in the lamb, some of the rabbit anti-sheep globulins were disappearing due to their binding by the continuous production of low levels of /22a-globulin normally found in the fetal lamb (5). Perhaps the chief reason for the rapid disappearance of the rabbit anti-sheep globulins, however, was initiation of antibody production by the fetus against one or more of the rabbit protein antigens injected, since each of these animals had plasma cells in its lymph nodes and spleen. Such antibody globulin response on the part of the fetus would tend to deplete its circulation more rapidly of rabbit anti-sheep globulin antibodies. These considerations suggest that during the earlier stages of graft rejection, 1173 Abstract. The x-ray diffraction patterns of normal myelin, and of myelin expanded by soaking in hypotonic solutions, have been compared quantitatively. Application of the "shrinkage stage" method for determining the course of a real Fourier transform allows all but 2 of the 32 possible sign combinations to be Abstract. The x-ray diffraction patterns of normal myelin, and of myelin expanded by soaking in hypotonic solutions, have been compared quantitatively. Application of the "shrinkage stage" method for determining the course of a real Fourier transform allows all but 2 of the 32 possible sign combinations to be
doi:10.1126/science.142.3596.1173 pmid:14069237 fatcat:ku2exvjbgfdlznbazqxqv7irde