3) Application of Radioisotopes to the Diagnosis of Anemias

Gyoichi WAKISAKA
1969 Japanese Journal of Medicine  
Radioisotopes are widely used for the diagnosis and elucidation of the pathogenesis of various anemias. In this paper the application of radioisotopes in this field was presented based on the experiences in our laboratory. 1) Studies of iron absorption by the simultaneous use of 59Fe and 55Fe A new analytical method for the study of iron absorption using 59Fe and 55Fe was described, and the absorption of iron from the intestinal tract was studied under various conditions. By this technique it
more » ... this technique it was demonstrated that the absorption of iron from the intestine could be divided into two phases, namely the rapid phase and slow phase. In patients with iron deficiency anemia, the increase of iron absorption was mainly due to the increase of iron absorption in the rapid phase. There was a statistically significant correlation between the iron absorption in the rapid phase and the serum iron level as well as the plasma iron disappearance rate. It was confirmed that ferrous iron was more easily absorbed than ferric iron, and the absorption of iron was increased by the simultaneous oral administration of ascorbic acid or reduced glutathione. 2) Studies of iron metabolism in the reticuloendothelial system by the simultaneous use of 59Fe and 55Fe The functions of the reticuloendothelial system with regard to iron metabolism, namely the uptake of colloidal iron and the release of iron, were separately investigated using chondroitin sulfate iron labelled with 59Fe and 55Fe globulinate. In iron deficiency anemia, the release of iron from the reticuloendothelial system following the intravenous administration of colloidal iron was increased as compared with normal controls, while in rheumatoid arthritis and acquired hemolytic anemia, it was decreased as compared with normal controls. In aplastic anemia it was demonstrated that the release of iron from the reticuloendothelial system following the injection of colloidal iron was increased by the administration of steroid hormone. 3) Determination of the red cell life span in various diseases by the simultaneous use of 51Cr and DF32P In normal controls, hemolytic anemia and aplastic anemia, the red cell life span measured by 51Cr technique was parallel to that measured by DF32P, while in iron deficiency anemia the red cell life span measured by 51Cr technique was longer than that measured by DF32P. This discrepancy was found to be due to the reduced elution rate of 51Cr in iron deficiency anemia. In iron deficiency anemia there was
doi:10.2169/internalmedicine1962.8.208 fatcat:cud7xcq7p5dy5lahcmvyuoqk4u