Background" The purpose of this research was to examine the salivary groups of sodium chloride, magnesium, and calcium of unstimulated whole spit in pregnant women in three trimesters and compare the characteristics of non-pregnant women. Methods A longitudinal investigation. At the Acharya Vinoba Bhave Rural Hospital in Wardha, India, 75 women who were pregnant or were in the process of becoming pregnant and 75 women who were not pregnant were included in the study's sample of 75 pregnant and

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... 5 non-pregnant women. Salivary Sodium, Chloride, Magnesium, and Ionic calcium concentrations were measured in unstimulated whole saliva. By using the Student t test and the ANCOVA, the data were examined (two-tailed α = 0.05). Results As the trimester progressed, the sodium, Chloride and ionic calcium concentrations continued to decline and magnesium levels were rising as the pregnancy progressed while the values in the non-pregnant women were in the normal range. Conclusion This research found differences in unstimulated whole saliva between pregnant and non-pregnant women.