Modification of a strain of Aujeszky's disease virus in cell culture

B. TOMA, A. BRUN, P. VANNIER, G. CHAPPUIS
1977 Annales de Zootechnie  
A virulent virus strain isolated in Brittany during an outbreak of the _lujeszky's diseasewas subjected to serial passages on cell culture, by the technique of limit dilutions, at a progressively decreasing temperature, and cloned three times. At passage 220 , the residual virulence and the immunogenicity of this strain (Alfort 2 6) were estimated. The preliminary results show that the strain is harmless for the 2 day-old piglet receiving it by the nasal, intramuscular or intracerebral route.
more » ... racerebral route. Furtherm.ore, it proves to be immunogenic for the weaned pig. These primary findings have to be completed by many further investigations.
doi:10.1051/animres:19770332 fatcat:gugywvlghne77hretkanermdk4