Comparison between ectodomain and G2 region of G glycoprotein for genotyping of HRSV

Angélica Cristine de Almeida Campos, Edison Luiz Durigon, Andréa Lima Leal, Tereza Souza Silva, Patrícia Alves Ramos Bosso, Claudia Trigo Pedroso Moraes, Danielle Bruna Leal Oliveira, Hildener Nogueira Lima, Sandra Elisabete Vieira, Viviane Fongaro Botosso, Paolo Marinho de Andrade Zanotto, Klaus Eberhard Stewien
2007 Brazilian Journal of Microbiology  
The Human Respiratory Syncytial Virus (HRSV), isolated in 1955, is the main cause of hospitalization of babies and infants with respiratory illness. Several studies have been conducted worldwide aiming the development of a safe and effective vaccine against HRSV. The G2 region of glycoprotein G is used as genotyping default. In the present study, we performed a phylogenetic analysis of G protein and a comparative study between G2 region and ectodomain of attachment glycoprotein. Fifty-three
more » ... in. Fifty-three nasal swab samples from children less than 5 years old and presenting symptoms of acute respiratory illness, assisted at the University Hospital (UH) of University of Sao Paulo (USP) in 2004, were submitted to sequencing by PCR and compared with GenBank sequences. We concluded that the G2 region is adequate for HRSV genotyping.
doi:10.1590/s1517-83822007000300005 fatcat:pq2sdh6inveffimzu3lobst37i