Nonlinear Dynamics of Heart Rate Variability after Superoxide Dismutase Inhibition in Rats

Stanislaw Zajaczkowski, Tomasz Wierzba
2016 2016 Computing in Cardiology Conference (CinC)   unpublished
Superoxide dismutase (SOD) is a crucial enzyme for endogenous oxidative balance. We tested how diethylthiocarbamate (DETC), a nonspecific SOD inhibitor and oxidant, influence heart rate variability (HRV). Fourteen Wistar rats (divided into two groups) with implanted subcutaneous ECG electrodes, maintained unrestrained in experimental setup. Time-series of 1024 RR intervals (RRi) were extracted from continuous ECG recording for HRV analysis. SOD activity was measured by dedicated assay kit.
more » ... ted assay kit. Intraperitoneal injection of DECT resulted in SOD inhibition by 30%. HRV analysis confirms a role of ROS in the regulation of sinoatrial rhythm. Increased HR after DETC injection was followed by a reduction in overall HRV including LF/HF. A significant reduction of the recurrence quantification analysis (RQA) parameters: %REC, %DET, DFAα1 and DFAα2 is indicative of some loss of complexity and selfsimilarity within the tested RRi time-series. The reduction of intrinsic harmonic correlations can be interpreted as a simplification of the regulatory feedback loops with more random heart rhythm poorly controlled by autonomic nervous system. Nonlinear dynamics methods such as RQA have a potential to increase the reliability of data in experimental studies.
doi:10.22489/cinc.2016.287-404 fatcat:gnddl6urubbp3nolos6rkzizaq