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The comparative efficacy of Rose Bengal Plate Test (RBPT) and Milk Ring test (MRT) was calculated in terms of sensitivity and specificity for the diagnosis of bovine brucellosis in cows (Group A) and buffaloes (Group B) from Lahore and Okara districts of Punjab, Pakistan. Using bacterial growth as a gold standard RBPT showed high sensitivity values of 100% in both groups. While its specificity was 96.29% (Group A) and 90.62% (Group B). On the other hands MRT showed low sensitivity (80.0% infatcat:pptozvknzjcgrccqvg5tpxc2be