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A new high performance liquid chromatographic method has been established for simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis. Analytical curves showed good linear regression in the concentration ranges 0.49-7.8 μg/mL for chlorogenic acid, 0.25-3.9 μg/mL for caffeic acid and 0.244-7.8 μg/mL for caffeine. Reduction of the DPPH· radical was used to determine the antioxidant capacity of the extracts. Our methoddoi:10.1590/s1516-93322007000200007 fatcat:trokm6mrtvbf7kar5ee7gimwui