Simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis by HPLC and correlation with antioxidant capacity of the extracts by DPPH· reduction

Diogo Pineda Rivelli, Vanessa Vitoriano da Silva, Cristina Dislich Ropke, Denise Varella Miranda, Rebeca Leite Almeida, Tania Cristina Higashi Sawada, Silvia Berlanga de Moraes Barros
2007 Revista Brasileira de Ciências Farmacêuticas  
A new high performance liquid chromatographic method has been established for simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis. Analytical curves showed good linear regression in the concentration ranges 0.49-7.8 μg/mL for chlorogenic acid, 0.25-3.9 μg/mL for caffeic acid and 0.244-7.8 μg/mL for caffeine. Reduction of the DPPH· radical was used to determine the antioxidant capacity of the extracts. Our method
more » ... for the simultaneous determination of chlorogenic acid, caffeic acid and caffeine was highly sensitive, having lower detection and quantitation limits than other papers that used similar methodology.
doi:10.1590/s1516-93322007000200007 fatcat:trokm6mrtvbf7kar5ee7gimwui