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Characterization of Proteolytic Activity in Trichomonas vaginalis Isolates from Brazilian Patients
1997
The Journal of protozoology research
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and200kDaonacidicpH,dependingontheisolates.The proteinasesbelongto CySteine−PrOteinase classes according toinhibition whenleupeptln WaS uSed as inhibitorinpresenceofDTT・Some serine−PrOteinasescouldalsobefbundand therewasinhjbitionoftheactivlty OftJle eXtraCtS,Since PMSFwas used・The extracts were passed throughanimmunoafhity−COlumn coupledwithantiLT. VaginalisIgGisolatedfromimmuneseraandtheproteaseactivlty Seemedtobe COnCentratedin the void・Tllisis the nrst time that proteinases of Brazilian
doi:10.32268/jprotozoolres.7.3_70
fatcat:2hiecvrpl5ekznssk6we6v7x74