Die Flexibilität von TAL-Effektoren in der Natur und als molekulare Werkzeuge zur Genaktivierung und Genommodifikation [article]

Annekatrin Richter, Alain Tissier, Sven-Erik Behrens, Jens Boch, Martin-Luther Universität, Universitäts- Und Landesbibliothek Sachsen-Anhalt
2020
TALEs are proteins of phytopathogenic Xanthomonas species. They bind to promoter sequences in the host plant cells and thereby induce genes. Their DNA-binding domain consists of 33 to 35 amino acid (aa) tandem repeats. Each repeat specifically recognizes one nucleotide in the target sequence. In this work, it was demonstrated that TALEs with an aberrant repeat of 30, 39, 40 or 42 aa recognize an optimal target sequence as well as one with a -1-nucleotide frameshift. This was used to generate
more » ... used to generate TALEs that efficiently induced transcription at several promoter variants. Furthermore, it was shown that artificial TALEs are able to cooperatively induce transcription together with endogenous transcription factors. Additionally, single chain TALE nucleases (scTALEN) were constructed as an alternative to TALEN pairs. Although efficiently cleaving DNA in vitro, these scTALEN did not induce mutations in living cells. Finally, TALEN-pairs with a single nucleotide specificity were generated.
doi:10.25673/33893 fatcat:rbk6movtofb5lbqxgeom4zolpu