Belliella buryatensis sp. nov., isolated from alkaline lake water

Lyudmila Kozyreva, Elena Plotnikova, Lyudmila Anan'ina, Darya Egorova, Larisa Radnaeva, Bair Namsaraev, Elena Ariskina, Natalya Prisyazhnaya
2016 International Journal of Systematic and Evolutionary Microbiology  
Two bacterial isolates from water of the alkaline brackish Lake Solenoe (Buryatia, Russia), 2C and 5C T , were characterized by using a polyphasic taxonomic approach. The strains were small, non-motile, Gram-stain-negative rods that formed small orange-red colonies on the surface of marine agar. Studies based on 16S rRNA gene sequences showed that the strains were related closely to Belliella pelovolcani CC-SAL-25 T (98.7 % sequence similarity). The G+C content of the DNA was 38-40 mol%.
more » ... hybridization values between strains 2C and 5C T and B. pelovolcani CC-SAL-25 T were 56-58 mol%. A menaquinone with seven isoprene units (MK-7) was the major respiratory quinone. The fatty acid profiles were slightly different from that of B. pelovolcani CC-SAL-25 T . The novel strains could be distinguished from the phylogenetically closest species B. pelovolcani CC-SAL-25 T based on matrix-assisted laser desorption ionization time-of-flight mass spectra of whole cells and a range of physiological and biochemical characteristics. The data obtained suggest that strains 2C and 5C T represent a novel species of the genus Belliella, for which the name Belliella buryatensis sp. nov. is proposed. The type strain is 5C T (5VKM B-2724 T 5KCTC 32194 T ). Abbreviation: MALDI-TOF, matrix-assisted laser desorption ionization time-of-flight. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 5C T is LK021687. Three supplementary tables are available with the online Supplementary Material.
doi:10.1099/ijsem.0.000682 pmid:26474559 fatcat:chx3we577rczzlq6tm7b32a3ti