Correct delimitation of taxonomic entities is essential in conservation biology and methods based on population geneticscan be particularly helpful where other tools fail to provide reliable conclusions. In this paper we use population genetics and AFLP markers to elucidate the taxonomic status of the enigmatic Villafuerte daffodil (Narcissus enemeritoi (Sánchez-Gómez et al.), a taxon known from a single, small population in southeastern Spain whose position within the complex Pseudonarcissus

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... ction of the genus Narcissus has proven elusive so far. Compared to populations from other four southeastern Spanish taxa, the Villafuerte daffodil population was genetically indistinguishable from Narcissus nevadensis subsp. longispathus (Degen and Hervier ex Pugsley) Algarra, Blanca, Cueto and Fuentes. Genetic diversity and singularity of its single population were unexpectedly high. Despite losing its taxonomic distinctiveness after being subsumed by the relatively abundant N. n. subsp. longispathus, genetic singularity and diversity of Villafuerte daffodil still justify continuation of current conservation efforts.