Micropropagation of Babiana Spp

B.G. McAlister, A.K. Jäger, J. van Staden
1998 South African Journal of Botany  
Seeds of six species of Babiana were germinated aseptically. Once germinated the seedlings were allowed to grow until 6 em in height and were then cut into pieces giving root, hypocotyl and leaf explants. These were placed on Murashige and Skoog medium containing 4.4 1-LM 6-benzylaminopurine and 5.3 1-LM 1-naphthaleneacetic acid. After 2-3 weeks multiple shoot formation occurred on the hypocotyl portion. Maximum shooting occurred on medium containing 8.8 and 22.1 1-LM 6-benzylaminopurine and no
more » ... 1-naphthaleneacetic acid. Optimal rooting occurred on a hormone free medium. After rooting, plants from all species were hardened off in vermiculite or Genu lite in a mist house for 3-4 weeks. Plants were then transplanted into a soil and sand mix (1 :2 ratio). The plants were s uccessfully hardened-off and flowered 6-8 months later
doi:10.1016/s0254-6299(15)30831-0 fatcat:gw5q2ezdnveyvarloyqt7qickm