Traumatic Brain Injury Induces Rapid Enhancement of Cortical Excitability in Juvenile Rats

Joshua Nichols, Roxy Perez, Chen Wu, P. David Adelson, Trent Anderson
2014 CNS Neuroscience & Therapeutics  
Following a traumatic brain injury (TBI) 5-50% of patients will develop post traumatic epilepsy (PTE). Pediatric patients are most susceptible with the highest incidence of PTE. Currently, we cannot prevent the development of PTE and knowledge of basic mechanisms are unknown. This has led to several shortcomings to the treatment of PTE, one of which is the use of anticonvulsant medication to the population of TBI patients that are not likely to develop PTE. The complication of identifying the
more » ... o populations has been hindered by the ability to find a marker to the pathogenesis of PTE. The central hypothesis of this dissertation is that following TBI, the cortex undergoes distinct cellular and synaptic reorganization that facilitates cortical excitability and promotes seizure development. Chapter 2 of this dissertation details excitatory and inhibitory changes in the rat cortex after severe TBI. This dissertation aims to identify cortical changes to a single cell level after severe TBI using whole cell patch clamp and electroencephalography (EEG). The work of this dissertation concluded that excitatory and inhibitory synaptic activity in cortical controlled impact (CCI) animals showed the development of distinct burst discharges that were not present in control animals. The results suggest that CCI induces early "silent" seizures that are detectable on EEG and correlate with changes to the synaptic excitability in the cortex. The synaptic changes and development of burst discharges may play an important role in synchronizing the network and promoting the development of PTE. ii ACKNOWLEDGEMENTS My appreciation for my advisor, Trent Anderson PhD, cannot be expressed enough. Trent has been an integral role of my education as a young scientist.
doi:10.1111/cns.12351 pmid:25475223 pmcid:PMC5880220 fatcat:gxp4k3vnhfcvbetijdxbboei4a