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In-gel screening of phosphorus and copper, zinc and iron in proteins of yeast mitochondria by LA-ICP-MS and identification of phosphorylated protein structures by MALDI-FT-ICR-MS after separation with two-dimensional gel electrophoresis
2004
Journal of Analytical Atomic Spectrometry
A new screening technique using two-dimensional gels was developed in order to rapidly identify various elements in well-separated protein spots. Yeast mitochondrial proteins were separated using two-dimensional gel electrophoresis (blue native/SDS 2D-PAGE) and marked by silver staining. The 2D gels were systematically analyzed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) using a double-focusing sector field instrument. From more than 60 mitochondrial protein spots
doi:10.1039/b404797b
fatcat:uen4hvsngzdupc6psesd24skfi