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Direct visual detection of aflatoxin synthesis by minicolonies of Aspergillus species
1989
Applied and Environmental Microbiology
Single-spore colonies of Aspergillus flavus and Aspergillus parasiticus, grown for 4 to 5 days at 25°C on a coconut extract agar containing sodium desoxycholate as a growth inhibitor, produced aflatoxin, readily detectable as blue fluorescent zones under long-wave (365 nm) UV light. Over 100 colonies per standard petri dish were scored for aflatoxin production by this procedure. Progeny from some strains remained consistently stable for toxin production after repeated subculture, whereas
doi:10.1128/aem.55.7.1808-1810.1989
fatcat:lpox5bdtdzeszkr52so76hcimm