The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase

Gary William Boyd, Marion Drew, Shannon Ward, Marianne Baird, Christopher Connaboy, Scott Murray Graham
2018 Applied Physiology, Nutrition and Metabolism  
Branched-chain amino acids (BCAA) are used as nutritional support for patients with a range of conditions including liver cirrhosis and in-born errors of amino acid metabolism, and are commonly used "sports"/exercise supplements. The effects of the BCAA on the in-vitro activity of calf intestinal alkaline phosphatase (EC. 3.1.3.1) were studied. All three BCAA were found to be uncompetitive inhibitors of the enzyme with L-leucine being the most potent (Ki ' = 24.9mM) and L-valine, the least
more » ... t (Ki' = 37mM). Mixed BCAA are able to act in combination to inhibit the enzyme. Given the important role of intestinal alkaline phosphatase in gut homeostasis, these findings have potential implications for those taking high levels of BCAA as supplements.
doi:10.1139/apnm-2018-0449 pmid:30398915 fatcat:c4whsqsbazeghiqoptdtvisrlq