Identification, crystallization and epitope determination of public TCR shared and expanded in COVID-19 patients [article]

Xiuyuan Lu, Yuki Hosono, Shigenari Ishizuka, Masamichi Nagae, Eri Ishikawa, Daisuke Motooka, Yuki Ozaki, Nicolas Sax, Ryo Shinnakasu, Takeshi Inoue, Taishi Onodera, Takayuki Matsumura (+12 others)
2021 bioRxiv   pre-print
T cells play pivotal roles in protective immunity against SARS-CoV-2 infection. Follicular helper T (Tfh) cells mediate the production of antigen-specific antibodies; however, T cell receptor (TCR) clonotypes used by SARS-CoV-2-specific Tfh cells have not been well characterized. Here, we first identified and crystallized public TCR of Tfh clonotypes that are shared and expanded in unhospitalized COVID-19-recovered patients. These clonotypes preferentially recognized SARS-CoV-2 spike (S)
more » ... epitopes which are conserved among emerging SARS-CoV-2 variants. These clonotypes did not react with S proteins derived from common cold human coronaviruses, but cross-reacted with symbiotic bacteria, which might confer the publicity. Among SARS-CoV-2 S epitopes, S864-882, presented by frequent HLA-DR alleles, could activate multiple public Tfh clonotypes in COVID-19-recovered patients. Furthermore, S864-882-loaded HLA tetramer preferentially bound to CD4+ T cells expressing CXCR5. In this study, we identified and crystallized public TCR for SARS-CoV-2 that may contribute to the prevention of COVID-19 aggravation.
doi:10.1101/2021.03.23.436573 fatcat:kvd7wyiifrgylm6aebrvpywxfi