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Identification, crystallization and epitope determination of public TCR shared and expanded in COVID-19 patients [article]

Xiuyuan Lu, Yuki Hosono, Shigenari Ishizuka, Masamichi Nagae, Eri Ishikawa, Daisuke Motooka, Yuki Ozaki, Nicolas Sax, Ryo Shinnakasu, Takeshi Inoue, Taishi Onodera, Takayuki Matsumura (+12 others)
2021 bioRxiv   pre-print

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T cells play pivotal roles in protective immunity against SARS-CoV-2 infection. Follicular helper T (Tfh) cells mediate the production of antigen-specific antibodies; however, T cell receptor (TCR) clonotypes used by SARS-CoV-2-specific Tfh cells have not been well characterized. Here, we first identified and crystallized public TCR of Tfh clonotypes that are shared and expanded in unhospitalized COVID-19-recovered patients. These clonotypes preferentially recognized SARS-CoV-2 spike (S)
more » ... epitopes which are conserved among emerging SARS-CoV-2 variants. These clonotypes did not react with S proteins derived from common cold human coronaviruses, but cross-reacted with symbiotic bacteria, which might confer the publicity. Among SARS-CoV-2 S epitopes, S864-882, presented by frequent HLA-DR alleles, could activate multiple public Tfh clonotypes in COVID-19-recovered patients. Furthermore, S864-882-loaded HLA tetramer preferentially bound to CD4+ T cells expressing CXCR5. In this study, we identified and crystallized public TCR for SARS-CoV-2 that may contribute to the prevention of COVID-19 aggravation.
doi:10.1101/2021.03.23.436573 fatcat:kvd7wyiifrgylm6aebrvpywxfi
Citation

Xiuyuan Lu, Yuki Hosono, Shigenari Ishizuka, Masamichi Nagae, Eri Ishikawa, Daisuke Motooka, Yuki Ozaki, Nicolas Sax, Ryo Shinnakasu, Takeshi Inoue, Taishi Onodera, Takayuki Matsumura, Masaharu Shinkai, Takashi Sato, Shota Nakamura, Shunsuke Mori, Teru Kanda, Emi E. Nakayama, Tatsuo Shioda, Tomohiro Kurosaki, Hisashi Arase, Kazuo Yamashita, Yoshimasa Takahashi, Sho Yamasaki. "Identification, crystallization and epitope determination of public TCR shared and expanded in COVID-19 patients." bioRxiv (2021)