Analysis of Metabolites from Purple Cleome Extract (Cleome rutidosperma Linn.) as Potential Organic Fungicides

Ali Ikhwan, Dian Indratmi, Faridlotul Hasanah, Manar Fayiz Mousa Atoum, Irum Iqrar
2021 Sarhad Journal of Agriculture  
One way to overcome these pathogens is to spray plant extract, e.g., purple cleome plant (spider or bee plant -Cleome rutidosperma Linn.) extracts as organic fungicides (Aliyu and Prasad, 2020) . Purple Cleome plant extract has a chemical compound, thiogluco-Abstract | One disease that often attacks chili (Capsicum L.) is anthracnose caused by Colletotrichum capsici [(Syd.) E.J. Butler & Bisby]. In the field, C. capsici is controlled with a chemical fungicide that harms the environment. The
more » ... le Cleome plant (Spider plant -Cleome rutidosperma Linn.) can be extracted and function as an organic fungicide for environment-friendly control. This research aimed to examine the type and concentration of purple cleome extract metabolites and understand their effectiveness in inhibiting C. capsici. Purple cleome leaf is extracted with 1:1 w/v absolute methanol and concentrated with 1:1 v/v cold absolute methanol, and then the supernatant is purified using a centrifuge. The metabolic analysis was performed using Gas Chromatography-Mass Spectrometry (GC-MS) and comparing the data with the National Center for Biotechnology Information (NCBI) database to obtain specific metabolites. The inhibition test was done with one control (P0: chemical fungicide) and two-level of treatment, i.e., the concentration of purple Cleome extract (P1: 80 % and P2: 40 %), then ANOVA and HSD test were carried out to find out the best treatment. The purple Cleome leaf extract contained three metabolite compounds as organic fungicides, namely propanoic acid 4.12 %, phenol 8.38 %, and isopropyl myristate 15.86 %. The inhibition test confirmed that 80 % (P2) purple cleome extract could suppress C. capsici attack up to 16.67 %, significantly higher than chemical fungicide (24.4 %).
doi:10.17582/journal.sja/2021/37.s1.115.121 fatcat:y52mtzojtfffjkrtz6gded5cey