The validity of the classification for lateral hinge fractures in open wedge high tibial osteotomy
Asia-Pacific Journal of Sports Medicine, Arthroscopy, Rehabilitation and Technology
stiffness of the knee under an anterior drawer force and the structural properties of the femurgraft-tibia complex were evaluated. Results: The remnant preservation significantly accelerated revascularization in the grafted tendon, and significantly increased the number of mechanoreceptors in the reconstructed ACL at 4 and 12 weeks. The remnant preservation significantly improved the anterior translation and the initial stiffness of the ACL-reconstructed knee in drawer testing at 12 weeks.
... g at 12 weeks. Discussion: Preservation of the remnant tissue in ACL reconstruction not only enhanced cell proliferation, revascularization, and regeneration of proprioceptive organs in the reconstructed ACL, but also improved the knee stability. Conclusion: Preservation of the remnant tissue may be beneficial in improving the clinical outcome of ACL reconstruction. Background: To investigate the effect of silk fibroin (SF) coating for the cell compatibility in vitro of polyethylene terephthalate (PET) material and for the ligamentization of intra-articular segment in vivo of PET artificial ligament. Methods: The surface of PET artificial ligament was treated by plasma, and then PET artificial ligament was soaked in an SF solution. BALB / C CL7 mice ligament fibroblasts was cultured in the uncoated-PET material (control group), SF coating-PET material (SF group). Subsequently, scanning electron microscope (SEM), methyl thiazolyl tetrazolium (MTT), cell cycle analysis, collagen protein and DNA content was detected. Furthermore, the mRNA expression levels of fibronectin (FN), b-actin, integrin a1 and integrin b1 were also detected. Intra-articular segment of PET artificial ligament was coated by SF, which was used to reconstruct Beagles's anterior cruciate ligament (ACL). The coverage of intra-articular synovial tissue covering the artificial ligament was assessed. Results: The results of SEM, MTT and cell cycle analysis showed SF group could significantly improve the proliferation of BALB/C CL7 mice ligament fibroblasts (P<0.05). Collagen protein and DNA content analysis showed that the specific surface area was increased by 3D structure in the SF group (P<0.05), which provides a good growth substrate for the high density culture of BALB/C CL7 mice ligament fibroblasts. MRNA expression analysis of FN, b-actin, integrin a1 and integrin b1 showed that the normal BALB/C CL7 mice ligament fibroblasts related gene expression was not changed in the PET-SF composite materials (P>0.05). One month after ACL reconstruction, synovial tissue coverage in the SF group and control group was 85.4 ± 4.3% and 62.8 ± 5.7%, respectively (P <0.05). Moreover, three months after ACL reconstruction, synovial tissue coverage in the SF group and control group was 97.7 ± 2.1%% and 92.5 ± 4.3%, respectively (P>0.05). Conclusion: PET-SF composite materials has good cell biocompatibility in vitro. ACL reconstruction in vivo confirmed that the SF coating could improve the coverage of intra-articular synovial tissue covering the artificial ligament early, which speeds up the process of ligamentization. http://dx.