Apoptosis of HeLa and CaSki cell lines incubated with All-trans retinoid acid

Dorota Darmochwal-Kolarz, Urszula Gasowska-Giszczak, Robert Paduch, Bogdan Kolarz, Piotr Wilciński, Jan Oleszczuk, Anna Kwasniewska
2010 Folia Histochemica et Cytobiologica  
The aim of the study was to evaluate the concentrations of a soluble form of APO-1/Fas antigen (sFas, CD95) and a soluble Ligand for APO-1/Fas antigen (sCD95L, sFasL) in supernatants from CaSki and HeLa cell line cultures after the incubation with All-trans-retinoic acid. HPV-16 and HPV18 -positive cell lines were cultivated with All-trans-retinoic acid in concentrations of 1×10 -6 M/L and 1×10 -8 M/L. The cultures were incubated for 24 hours. Control culture with 3 μl of dimethyl-sulphoxide
more » ... SO) was incubated under identical conditions. The concentrations of soluble APO-1/Fas antigen and Fas Ligand in cell culture supernatants were estimated using immunoenzymatic methods. The obtained results showed significant decrease of concentrations of soluble APO-1/Fas antigen in supernatants from HeLa cell lines incubated with retinol in comparison with the control culture. Moreover, the concentrations of soluble Ligand for APO-1/Fas antigen in the supernatants of CaSki and HeLa cell lines were significantly lower in the culture incubated with All-trans retinoid acid when compared to the control culture. Higher concentrations of soluble APO-1/Fas antigen in supernatants from HeLa cell line without retinol may constitute a protective mechanism of the cells infected with the virus before undergoing Fas/FasL-dependent apoptosis. Lower concentrations of soluble APO-1/Fas antigen and soluble Ligand for APO-1/Fas in the supernatants from CaSki and HeLa cell cultures incubated with retinol suggest that retinoids can decrease the synthesis of soluble APO-1//Fas and soluble FasL in HPV-16 and HPV -18 positive cells and that mechanisms protecting infected cells against Fas/FasL-mediated apoptosis become defective under the influence of retinol.
doi:10.2478/v10042-009-0113-0 pmid:20430726 fatcat:b7wikerh6vabfihlfhaopx5sce