Mobilization, Cloning, and Sequence Determination of a Plasmid-Encoded Polygalacturonase from a PhytopathogenicBurkholderia(Pseudomonas)cepacia

Carlos F. Gonzalez, Elizabeth A. Pettit, Victoria A. Valadez, Ellen M. Provin
1997 Molecular Plant-Microbe Interactions  
Phytopathogenic strains of Burkholderia cepacia (synonym Pseudomonas cepacia) produce endopolygalacturonase, whereas strains of clinical and soil origin do not. Growth of a phytopathogenic strain (ATCC25416) at elevated temperatures resulted in nonpectolytic derivatives that were either cured of a resident plasmid or contained a plasmid of reduced mass. The resident 200-kb plasmid (pPEC320) in strain ATCC25416 was tagged with Tn5-Mob. The pPEC320::Tn5-Mob (pPEC321) plasmid was mobilized in B.
more » ... s mobilized in B. cepacia strains of soil and clinical origin. Transconjugants containing pPEC321 expressed the endopolygalacturonase and showed differential activity on plant tissue. No evidence for self-transfer of pPEC320 or the tagged derivative was observed. A 2.85-kb cloned fragment from pPEC320 containing the plasmid-borne pehA gene was sequenced and compared to the pehA gene from Erwinia carotovora subsp. carotovora and Ralstonia solanacearum and the polygalacturonase sequence from Lycopersicon esculentum.
doi:10.1094/mpmi.1997.10.7.840 pmid:9304858 fatcat:etuwmmc4hfhjtcfu2nng7mrlam