ANTIGENIC STRUCTURE OF ACTINOMYCETALES VI

J. B. Kwapinski
1963 Journal of Bacteriology  
KWAPINSKI, J. B. (University of New England, Armidale, N.S.W., Australia). Antigenic structure of Actinomycetales. VI. Serological relationships between antigenic fractions of Actinomyces and Nocardia. J. Bacteriol. 86:179-186. 1963.--A total of 52 chemical fractions were obtained by a comprehensive technique of preparation from three strains of Actinomyces and three strains of Nocardia. The chemical and serological structures and specificities of disintegrated cells, cell walls, cytoplasms,
more » ... lls, cytoplasms, and individual fractions were thoroughly studied. Cytoplasmic materials were found to be serologically alike or identical. The polysaccharide fractions, extracted from cell walls with alkali, formamide, and phenol, proved to be serologically related. Fractions prepared from the Nocardia by extractions in hot and concentrated solutions of acetic acid and sodium hydroxide, as well as the second protein fraction and the acetate-extracted polysaccharides of both the Nocardia and Actinomyces, proved to be genus-specific. An apparent morphological similarity between the Actinomyces and Nocardia was regarded by certain investigators as sufficient to classify these microorganisms as one or two genera within the family Actinomycetaceae (Wilson and Miles, 1957; Waksman and Henrici, 1943; Breed, Murray, and Smith, 1957) . Other investigators (Gordon and Mihm, 1957) divided strains of Nocardia into two different groups on the basis of colonial morphology, one being similar to Streptomyces and the other to Mycobacterium. Some differences in the chemical composition of cell walls, revealed by a chromatographic technique, were used by Cummins and Harris (1958) for the proposed taxonomic separation of Actinomyces from Nocardia, with the insertion of London; N. asteroides 5, obtained from and N. rubra, obtained from N. M. McClung, University of Georgia, Athens. Each strain was cultivated in 5% glycerol broth or a semisynthetic medium containing 0.25 % arabinose, 0.25% xylose, 1 % galactose, 179 on May 9, 2020 by guest
doi:10.1128/jb.86.2.179-186.1963 fatcat:k2fnfh5cobhndgf5cjrabgam2u